EDITOR-IN-CHIEF
Professor,Department of Education
GC University Faisalabad
Pakistan.
Email: khudabakhsh@gcuf.edu.pk
editorinchiefijmasri@gmail.com
Publisher
KRISHMA PUBLICATION (Reg 17/2020)
2 /57 A,
Middle street, Pali, Pali, Viluppuram,
Ulundurpettai, Tamil Nadu,
606104
Email: krishmapublication@gmail.com
EFFECT OF 2,4-D AND NAA ON SOMATIC EMBRYOGENESIS FROM APICAL PORTION OF GROUNDNUT (ARACHIS HYPOGAEA L.)
Somatic embryogenesis was carried out epicotyl portion of the mature embryo/apical portion. The somatic embryo induction medium containing 2,4-D or NAA (10.0 to 50.0 mg/l). Of the two concentrations tested 2,4-D (30.0mg/l) recorded the highest percentage of response followed by NAA (30.0mg/l). But the highest number of somatic embryo were recorded in 30.0mg/l of 2,4-D followed by NAA. The apical portion of the mature embryo formed direct embryos without any intervention of callus. The maximum percentage of embryogenic cultures were noticed in 30.0mg/l of 2,4-D followed by NAA at 30.0mg/l. for the differentiation of somatic embryos, the embryogenic masses were transferred to medium without any growth regulator. The maximum number of somatic embryos per culture was recorded in 30 mg/l of 2,4-D followed by 30.0 mg/l of NAA.
Keywords: Arachis hypogaea L.,Somatic Embryogenesis, 2,4-D and NAA
- 2021-09-06 14:24:41 Subject Area - Botany
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